2022
Application of Affitins for Affinity Purification of Proteins
Mouratou B, Pecorari F
Methods Mol Biol. 2022;2466:37-48. doi: 10.1007/978-1-0716-2176-9_3
2021
A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening
Vaurs J, Douchin G, Echasserieau K, Oger R, Jouand N, Fortun A, Hesnard L, Croyal M, Pecorari F, Gervois N, Bernardeau K
Sci Rep. 2021 Aug 26;11(1):17234. doi: 10.1038/s41598-021-96560-9
2020
Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins
Kalichuk V, Kambarev S, Béhar G, Chalopin B, Renodon-Cornière A, Mouratou B, Pecorari F
Methods Mol Biol. 2020;2070:19-41. doi: 10.1007/978-1-4939-9853-1_2
SPECT-CT Imaging of Dog Spontaneous Diffuse Large B-Cell Lymphoma Targeting CD22 for the Implementation of a Relevant Preclinical Model for Human
Etienne F, Berthaud M, Nguyen F, Bernardeau K, Maurel C, Bodet-Milin C, Diab M, Abadie J, Gouilleux-Gruart V, Vidal A, Bourgeois M, Chouin N, Ibisch C, Davodeau F
Front Oncol. 2020 Feb 7;10:20. doi: 10.3389/fonc.2020.00020
Characterization of Affitin proteolytic digestion in biorelevant media and improvement of their stabilities via protein engineering
Loussouarn A, Béhar G, Pecorari F, Croyal M, Renodon-Cornière A
Sci Rep. 2020 Nov 12;10(1):19703. doi: 10.1038/s41598-020-76855-z
2019
Model Affitin and PEG modifications onto siRNA lipid nanocapsules: cell uptake and in vivo biodistribution improvements
Resnier P, Lepeltier E, Emina AL, Galopin N, Bejaud J, David S, Ballet C, Benvegnu T, Pecorari F, Chourpa I, Benoit JP, Passirani C
RSC Adv. 2019 Aug 30;9(47):27264-27278. doi: 10.1039/c9ra03668g
Multivalent Affidendrons with High Affinity and Specificity towards Staphylococcus aureus as Versatile Tools for Modulating Multicellular Behaviors
Vukojicic P, Behar G, Tawara MH, Fernandez-Villamarin M, Pecorari F, Fernandez-Megia E, Mouratou B
ACS Appl Mater Interfaces. 2019 Jun 19;11(24):21391-21398. doi: 10.1021/acsami.9b05702
Whole-bacterium ribosome display selection for isolation of highly specific anti-Staphyloccocus aureus Affitins for detection- and capture-based biomedical applications
Béhar G, Renodon-Cornière A, Kambarev S, Vukojicic P, Caroff N, Corvec S, Mouratou B, Pecorari F
Biotechnol Bioeng. 2019 Aug;116(8):1844-1855. doi: 10.1002/bit.26989
Prosthetic groups for radioiodination and astatination of peptides and proteins: A comparative study of five potential bioorthogonal labeling strategies
Navarro L, Berdal M, Chérel M, Pecorari F, Gestin JF, Guérard F
Bioorg Med Chem. 2019 Jan 1;27(1):167-174. doi: 10.1016/j.bmc.2018.11.034
2018
A novel, smaller scaffold for Affitins: Showcase with binders specific for EpCAM
Kalichuk V, Renodon-Cornière A, Béhar G, Carrión F, Obal G, Maillasson M, Mouratou B, Préat V, Pecorari F
Biotechnol Bioeng. 2018 Feb;115(2):290-299. doi: 10.1002/bit.26463Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus
Kambarev S, Pecorari F, Corvec S
J Antimicrob Chemother. 2018 May 1;73(5):1201-1205. doi: 10.1093/jac/dky016Production de protéines recombinantes
Bernardeau K, Echasserieau K, Douchin G and Pecorari F
In “Principes des techniques de biologie moléculaire et génomique” (2018)
Coordinateed by Tagu D, Jaubert−Possamai S, Méreau A
Edited by Quae, collection “Savoir−faire”, ISBN : 978−2−7592−2885−0
2017
In vitro emergence of fluoroquinolone resistance in Cutibacterium (formerly Propionibacterium) acnes and molecular characterization of mutations in the gyrA gene
Takoudju EM, Guillouzouic A, Kambarev S, Pecorari F, Corvec S
Anaerobe. 2017 Oct;47:194-200. doi: 10.1016/j.anaerobe.2017.06.005Draft Genome Sequence of Mycobacterium ulcerans S4018 Isolated from a Patient with an Active Buruli Ulcer in Benin, Africa
Kambarev S, Corvec S, Chauty A, Marion E, Marsollier L, Pecorari F
Genome Announc. 2017 Apr 27;5(17). pii: e00248-17. doi: 10.1128/genomeA.00248-17Draft Genome Sequences of Two Highly Erythromycin-Resistant Streptococcus gallolyticus subsp. gallolyticus Isolates Containing a Novel Tn916-Like Element, Tn6331.
Kambarev S, Pecorari F, Corvec S
Genome Announc. 2017 Apr 20;5(16). pii: e00226-17. doi: 10.1128/genomeA.00226-17
2016
The archaeal “7 kDa DNA-binding” proteins: extended characterization of an old gifted family
Kalichuk V‡, Béhar G‡, Renodon-Cornière A, Danovski G, Obal G, Barbet J, Mouratou B, Pecorari F
Sci Rep. 2016 Nov 17;6:37274. doi: 10.1038/srep37274
‡Contributed equallyAffitins for protein purification by affinity magnetic fishing
Fernandes CS, Dos Santos R, Ottengy S, Viecinski AC, Béhar G, Mouratou B, Pecorari F, Roque AC
J Chromatogr A. 2016 Jul 29;1457:50-8. doi: 10.1016/j.chroma.2016.06.020Affitins as robust tailored reagents for affinity chromatography purification of antibodies and non-immunoglobulin proteins
Béhar G, Renodon-Cornière A, Mouratou B, Pecorari F
J Chromatogr A. 2016 Apr 8;1441:44-51. doi: 10.1016/j.chroma.2016.02.068
2015
Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes
Pacheco S, Cantón E, Zuñiga-Navarrete F, Pecorari F, Bravo A, Soberón M
AMB Express. 2015 Dec;5(1):73. doi: 10.1186/s13568-015-0160-1Biotechnological Uses of Archaeal Proteins
Pecorari F, Arcus VL, Wiegel J
Archaea. 2015 Oct 12;2015:809758. doi: 10.1155/2015/809758. eCollection 2015Draft Genome Sequence of Erythromycin-Resistant Streptococcus gallolyticus subsp. gallolyticus NTS 31106099 Isolated from a Patient with Infective Endocarditis and Colorectal Cancer
Kambarev S, Caté C, Corvec S, Pecorari F
Genome Announc. 2015 Apr 23;3(2). pii: e00370-15. doi: 10.1128/genomeA.00370-15Artificial affinity proteins as ligands of immunoglobulins
Mouratou B, Béhar G, Pecorari F
Biomolecules. 2015 Jan 30;5(1):60-75. doi: 10.3390/biom5010060
2014
Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability
Béhar G, Pacheco S, Maillasson M, Mouratou B, Pecorari F
J Biotechnol. 2014 Dec 20;192 Pt A:123-9Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR
Pacheco S, Béhar G, Maillasson M, Mouratou B, Pecorari F
Protein Eng Des Sel. 2014 Oct;27(10):431-8. doi: 10.1093/protein/gzu042Potent and specific inhibition of glycosidases by small artificial binding proteins (Affitins)
Correa A‡, Pacheco S‡, Mechaly AE, Obal G, Béhar G, Mouratou B, Oppezzo P, Alzari PM, Pecorari F
PLoS One. 2014 May 13;9(5):e97438. doi: 10.1371/journal.pone.0097438. eCollection 2014
‡Contributed equally
2013
Tolerance of the archaeal Sac7d scaffold protein to alternative library designs: characterization of anti-immunoglobulin G Affitins
Béhar G, Bellinzoni M, Maillasson M, Paillard-Laurance L, Alzari PM, He X, Mouratou B, Pecorari F
Protein Eng Des Sel. 2013 Apr;26(4):267-75. doi: 10.1093/protein/gzs106
2012
Engineering of a phosphorylatable tag for specific protein binding on zirconium phosphonate based microarrays
Cinier M, Petit M, Pecorari F, Talham DR, Bujoli B, Tellier C
J Biol Inorg Chem. 2012 Mar;17(3):399-407. doi: 10.1007/s00775-011-0863-yRibosome display for the selection of Sac7d scaffolds
Mouratou B, Béhar G, Paillard-Laurance L, Colinet S, Pecorari F
Methods Mol Biol. 2012;805:315-31. doi: 10.1007/978-1-61779-379-0_18
2011
Reagentless fluorescent biosensors from artificial families of antigen binding proteins
Miranda FF, Brient-Litzler E, Zidane N, Pecorari F, Bedouelle H
Biosens Bioelectron. 2011 Jun 15;26(10):4184-90. doi: 10.1016/j.bios.2011.04.030
2009
Bisphosphonate adaptors for specific protein binding on zirconium phosphonate-based microarrays
Cinier M, Petit M, Williams MN, Fabre RM, Pecorari F, Talham DR, Bujoli B, Tellier C
Bioconjug Chem. 2009 Dec;20(12):2270-7Llama VHH antibody fragments against GFAP: better diffusion in fixed tissues than classical monoclonal antibodies
Perruchini C, Pecorari F, Bourgeois JP, Duyckaerts C, Rougeon F, Lafaye P
Acta Neuropathol. 2009 Nov;118(5):685-95. doi: 10.1007/s00401-009-0572-6Type II secretion system secretin PulD localizes in clusters in the Escherichia coli outer membrane
Buddelmeijer N, Krehenbrink M, Pecorari F, Pugsley AP
J Bacteriol. 2009 Jan;191(1):161-8. doi: 10.1128/JB.01138-08
2008
Artificial binding proteins (Affitins) as probes for conformational changes in secretin PulD
Krehenbrink M, Chami M, Guilvout I, Alzari PM, Pecorari F, Pugsley AP
J Mol Biol. 2008 Nov 28;383(5):1058-68. doi: 10.1016/j.jmb.2008
2007
Remodeling a DNA-binding protein as a specific in vivo inhibitor of bacterial secretin PulD
Mouratou B, Schaeffer F, Guilvout I, Tello-Manigne D, Pugsley AP, Alzari PM, Pecorari F
Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):17983-8
2006
OB-fold used as scaffold for engineering new specific binders
Pecorari F and Alzari PM (2008)
Patent Publication Nos. PCT/IB2007/004388